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| 产品名称 | Recombinant Human KGF | 蛋白编号 | P21781 |
| 产品别称 | Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF | ||
| 产品描述 | Recombinant Human Fibroblast Growth Factor 7/Keratinocyte Growth Factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed. | ||
| 分子量 | Mol Mass:18.9 KDa; APMol Mass:17 KDa, reducing conditions | 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris，1mM EDTA，5% Trehalose, 0.02% Tween 80, pH 8.0. |
| 内毒素 | < 1 EU/µg as determined by LAL test. | 纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| 生物活性 | Human breast cancer organoids were cultured with Wnt3a, Noggin , R-spondin 3 , KGF, FGF-10 , EGF and NRG1-beta 1. The organoids showed good morphology. | ||
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| 保存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| 产品背景 | Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Immobilized Human KGF at 5 μg/mL (100 μL/well) can bind Human FGFR2 beta IIIb (C-Fc). The EC50 of Human FGFR2 beta IIIb (C-Fc) is 83 ng/mL.(Regularly tested).