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说明书下载产品属性/ Product Details
| 产品名称 | Recombinant Human NovoNectin | 蛋白编号 | P02751 |
| 产品别称 | NovoNectin; Fibronectin; FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1 | ||
| 产品描述 | Recombinant Human Fibronectin Fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed. | ||
| 分子量 | Mol Mass:62.7 KDa; APMol Mass:60-80 KDa, reducing conditions | 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 12.5 mM Citric acid, 1.25% Sucrose, 0.1% Tween80, pH 5.5 . |
| 内毒素 | < 0.01 EU/µg as determined by LAL test. | 纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| 生物活性 | Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 μg/ml. | ||
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| 保存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| 产品背景 | Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result. | ||
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Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
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Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 μg/ml.