当前位置：
说明书下载产品属性/ Product Details
| 产品名称 | Recombinant Human FLT3LG (C-6His) | 蛋白编号 | P49771 |
| 产品别称 | Fms-Felated Tyrosine Kinase 3 Ligand; Flt3 Ligand; Flt3L; SL Cytokine; FLT3LG | ||
| 产品描述 | Recombinant Human Fms-like Tyrosine Kinase 3 Ligand is produced by our Mammalian expression system and the target gene encoding Thr27-Pro184 is expressed with a 6His tag at the C-terminus. | ||
| 分子量 | Mol Mass:19 KDa; APMol Mass:24-32 KDa, reducing conditions | 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| 内毒素 | < 0.01 EU/µg as determined by LAL test. | 纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| 生物活性 | N/A | ||
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | ||
| 保存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. | ||
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. | ||
| 产品背景 | Fms-Related Tyrosine Kinase 3 Ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. Mature human Flt‑3 Ligand consists of a extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a transmembrane segment, and a cytoplasmic tail. Human and mouse Flt‑3 Ligand show cross-species activity. Flt‑3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts.It is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors by activiation of Flt 3 receptor. | ||
-
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
-
Immobilized Human FLT3LG (C-6His) at 5 μg/ml (100 μl/well) can bind Human FLT3 (C-Fc). The EC50 of Human FLT3 (C-Fc) is not higher than 10 ng/ml.