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| 产品名称 | β-tubulin Mouse Monoclonal Antibody(5G3)-HRP Conjugated | 反应种属 | H, R, M, Mk, Dg, C, Hm, Rb, Sh Insect, Yeast |
| 应用 | WB, IHC | ||
| 配方 | PBS, pH 7.4, 50% Glycerol. Avoid SODIUM AZIDE. | ||
| 别称 | Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain | ||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB: 1:5,000 IHC:1:200 Optimal dilutions should be determined by the end user. | ||
| 特异性 | Theβ-tubulin antibody can detects endogenousβ-tubulin protein. | ||
| 背景信息 | Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. | ||
| 分子量 | 55KD | 同型 | IgG1 |
| GeneID | 203068 | 理论分子量 | 55 |
| SWISS | P07437 | 宿主物种 | Mouse |
| 免疫原 | Recombinant Protein | 克隆性 | Monoclonal |
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Western blot analysis of Hela, with β-tubulin-HRP Conjugated diluted at 1:10,000, 20,000, 40,000, 80,000, 160,000, 320,000.