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货号:RCA20
β-actin Mouse Monoclonal Antibody(5B7)

规格:

50 μL

100 μL

价格:

¥500.00

¥900.00

货期: 3个工作日

产品属性/ Product Details
产品名称 β-actin Mouse Monoclonal Antibody(5B7) 反应种属 H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh Insect
应用 WB, IHC, IF
配方 PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
别称
储存 -20°C. Do not aliquot the antibody.
推荐稀释比 WB: 1:5,000 IHC: 1:200 IF: 1:200 Optimal dilutions should be determined by the end user.
特异性 Antibody can detects endogenous β-actin protein.
背景信息 β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
分子量 43KD 同型 IgG1
GeneID 60 理论分子量 43
SWISS P60709 宿主物种 Mouse
免疫原 Synthetic Peptide 克隆性 Monoclonal
  • Western blot analysis of Hela (1) Rat brain (2)Mouse brain (3) Chicken lung (4) and Rabbit testis (5)with β-Actin mouse mAb(5B7) diluted at 1:10,000.
  • IHC Staining of Human ovary tissue with β-Actin mouse mAb(5B7) diluted at 1:200