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| 产品名称 | GAPDH Mouse Monoclonal Antibody(2B8) | 反应种属 | H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh Insect, Yeast |
| 应用 | WB, IHC | ||
| 配方 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| 别称 | |||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB: 1:5,000 IHC: 1:200 Optimal dilutions should be determined by the end user. | ||
| 特异性 | Antibody can detects endogenous GAPDH protein. | ||
| 背景信息 | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. | ||
| 分子量 | 37KD | 同型 | IgG1 |
| GeneID | 2597 | 理论分子量 | 37 |
| SWISS | P04406 | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb(2B8) diluted at 1:10,000.
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Western blot analysis of Chicken Muscle (1), Hamster Lung (2), Pig Heart(3), with GAPDH mouse mAb(2B8) diluted at 1:10,000.