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| 产品名称 | PCNA Mouse Monoclonal Antibody(12D10) | 反应种属 | H, R, M |
| 应用 | WB, IHC | ||
| 配方 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| 别称 | |||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB: 1:5,000 IHC: 1:200 Optimal dilutions should be determined by the end user. | ||
| 特异性 | Antibody can detects endogenous PCNA protein. | ||
| 背景信息 | PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. | ||
| 分子量 | 29KD | 同型 | IgG1 |
| GeneID | 5111 | 理论分子量 | 29 |
| SWISS | P12004 | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T(4) with PCNA mouse mAb(12D10) diluted at 1:5000.
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IHC staining of Human breast cancer tissue paraffin-embedded with PCNA mouse mAb（12D10）diluted at 1:200