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| 产品名称 | β-actin Mouse Monoclonal Antibody (A02) | 反应种属 | H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh |
| 应用 | WB, IHC, IF | ||
| 配方 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| 别称 | actin antibody, b-actin antibody, beta-actin antibody | ||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB: 1:5,000-10,000 IHC: 1:200 IF: 1:200 Optimal dilutions should be determined by the end user. | ||
| 特异性 | Antibody can detects endogenous β-actin protein. | ||
| 背景信息 | β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues. | ||
| 分子量 | 43KD | 同型 | IgG1 |
| GeneID | 60 | 理论分子量 | 43 |
| SWISS | P60709 | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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Western blot analysis of Hela (1), Rat brain (2), Mouse brain(3), with β-actin mouse mAb(A02) diluted at 1:10,000.
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IF analysis of Hela with β-actin mouse mAb(A02) diluted at 1:500.