规格:
50 μL
100 μL
价格:
¥500.00
¥900.00
货期: 3个工作日
产品名称 | Flag Mouse Monoclonal Antibody(2C5)-HRP conjugated | 反应种属 | N/A |
应用 | WB | ||
配方 | PBS, pH 7.4, containing 0.02% Kathon as Preservative and 50% Glycerol. | ||
别称 | DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag | ||
储存 | -20°C. Do not aliquot the antibody. | ||
推荐稀释比 | WB: 1:5,000-10,000 Optimal dilutions should be determined by the end user. | ||
特异性 | The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. | ||
背景信息 | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. | ||
分子量 | N/A | 同型 | IgG1 |
GeneID | N/A | 理论分子量 | N/A |
SWISS | N/A | 宿主物种 | Mouse |
免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |