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| 产品名称 | Flag-Tag Mouse Monoclonal Antibody(2C5) | 反应种属 | N/A |
| 应用 | WB, IP, IF | ||
| 配方 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| 别称 | |||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB: 1:5,000 IP: 1:200 IF: 1:2000 Optimal dilutions should be determined by the end user. | ||
| 特异性 | The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins. | ||
| 背景信息 | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. | ||
| 分子量 | N/A | 同型 | IgG1 |
| GeneID | N/A | 理论分子量 | N/A |
| SWISS | N/A | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.
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IF analysis of 293 cells transfected with a Flag-tag protein, using anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)