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| 产品名称 | VSV-G-Tag Mouse Monoclonal Antibody(8D6) | 反应种属 | N/A |
| 应用 | WB, IP, IF | ||
| 配方 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| 别称 | |||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB: 1:5,000 IP: 1:200 IF: 1:1,000 Optimal dilutions should be determined by the end user. | ||
| 特异性 | The VSV-G tag antibody can recognize C-terminal, internal, and N-terminal VSV-G fusion proteins. | ||
| 背景信息 | Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation. | ||
| 分子量 | N/A | 同型 | IgG1 |
| GeneID | N/A | 理论分子量 | N/A |
| SWISS | N/A | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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1ug VSV-G fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000
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IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G)