植物脱落酸(ABA)酶联免疫吸附试剂盒-瑞迪生物科技（武汉）有限公司

产品名称	Plant Abscisic acid (ABA) ELISA Kit	反应种属	Plant
Uniprot ID	N/A	别称	ABA
检测方法	Competitive	灵敏度	0.09 ng/mL
标准品	16.2 ng/mL	检测范围	0.2-16.2 ng/mL
样品类型	Extract of plant tissue samples
检测时间	1H	研究领域	Enzyme & Kinase;
测试原理	This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ABA. During the reaction, ABA in the sample or standard competes with a fixed amount of ABA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ABA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ABA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

技术数据/ Technical Data

由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。

精密度

板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。

板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

回收率

分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。

线性

分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。