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| 产品名称 | β-Tubulin Mouse Monoclonal Antibody(5G3) | 反应种属 | H, R, M, Mk, Dg, C, Hm, Rb, Sh Insect, Yeast |
| 应用 | WB, IHC, IF | ||
| 配方 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| 别称 | |||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB: 1:5,000 IHC: 1:200 IF: 1:100-200 Optimal dilutions should be determined by the end user. | ||
| 特异性 | Antibody can detects endogenous β-tubulin protein. | ||
| 背景信息 | Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues. | ||
| 分子量 | 55KD | 同型 | IgG1 |
| GeneID | 203068 | 理论分子量 | 55 |
| SWISS | P07437 | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(5G3) diluted at 1:5000.
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IHC Staining of Human colon tissue with β-tubulin mouse mAb(5G3) diluted at 1:200.
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IF analysis of Hela with β-tubulin mouse mAb(Left) and DAPI (Right) diluted at 1:100.