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说明书下载产品属性/ Product Details
| 产品名称 | PCNA Mouse Monoclonal Antibody(7D1) | 反应种属 | H, M, R |
| 应用 | WB, IHC | ||
| 配方 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. | ||
| 别称 | Proliferating cell nuclear antigen | ||
| 储存 | -20°C. Do not aliquot the antibody. | ||
| 推荐稀释比 | WB 1:500-2,000 IHC：1:100~200 Optimal dilutions should be determined by the end user. | ||
| 特异性 | The antibody detects endogenous PCNA proteins. | ||
| 背景信息 | PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. | ||
| 分子量 | 29KD | 同型 | IgG1 |
| GeneID | 5111 | 理论分子量 | 29 |
| SWISS | P12004 | 宿主物种 | Mouse |
| 免疫原 | Synthetic Peptide | 克隆性 | Monoclonal |
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Western blot analysis of 1) 293T Cell Lysate, 2) Hela Cell Lysate, 3) 3T3 Cell Lysate, 4) Mouse Brain Tissue Lysate using PCNA Mouse mAb diluted at 1:5000.
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Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using PCNA Mouse mAb diluted at 1:200.